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High resolution respirometry The rate of oxygen consumption in the kidney and liver were measured using the Oroboros O2k system (Oroboros Instruments, Innsbruck, Austria) in animals 24 h after sham or AKI surgeries using a stepwise protocol to evaluate various components of the electron transport system

Recombinant mouse HGF (carrier protein-free) was incubated with GSSSG (A) and LASSS (B) at molar ratios ranging from 1:0 to 1:4000 relative to HGF (corresponding to weight ratios of 1:0 to 1:57 and 1:0 to 1:21 with A and B, respectively) just prior to the optimized peroxynitrite-protocol at a molar ratio 1:2000 relative to HGF, pH 7.4 (Figure 1b,c)

NICE guidelines (NG49) emphasise that weight loss, dietary changes, and increased physical activity are the most effective interventions for improving liver health in NAFLD